comparative molecular and microbiologic diagnosis of vaginal colonization by group b streptococcus in pregnant women during labor
Authors
abstract
objective(s) rapid tests for detection of streptococcus agalactiae or group b streptococci (gbs) at the onset of labor are needed to permit early intrapartum antibiotic prophylaxis. this study aimed to evaluate the pcr assays targeting the 16s ribosomal rna gene (16s rdna) for detection of the gbs in comparison with a specific culture method. materials and methods two swabs were used to obtain vaginal specimens from the 330 pregnant women attended delivery room at hedayat hospital, tehran, iran. one swab was analyzed by direct plating onto selective gbs agar medium (islam) and the other swab was used for a pcr assay, which amplified the 16s rdna of s. agalactiae. comparative study between the selective culture and the pcr assay was done among the 330 tested women. results the gbs colonization rate based on the culture results was 20.6% (68/330). both culture and pcr methods were positive for 56 and negative for 253 women. the culture method was positive and pcr was negative in 12 women. the culture was negative and the pcr positive for 9 women. sensitivity of the pcr assay was 82.3% and specificity was 96.5%. the positive predictive value was 86.15% and negative predictive value was 95.4%. conclusion islam diagnostic procedure and pcr are rapid and reliable analyzing methods, which might be useful for accurate diagnosis of gbs colonization in pregnant women at the time of delivery.
similar resources
Comparative Molecular and Microbiologic Diagnosis of Vaginal Colonization by Group B Streptococcus in Pregnant Women during Labor
Objective(s) Rapid tests for detection of Streptococcus agalactiae or Group B Streptococci (GBS) at the onset of labor are needed to permit early intrapartum antibiotic prophylaxis. This study aimed to evaluate the PCR assays targeting the 16S ribosomal RNA gene (16S rDNA) for detection of the GBS in comparison with a specific culture method. Materials and Methods Two swabs were used to obta...
full textComparative Molecular and Microbiologic Diagnosis of Vaginal Colonization by Group B Streptococcus in Pregnant Women during Labor
Objective(s) Rapid tests for detection of Streptococcus agalactiae or Group B Streptococci (GBS) at the onset of labor are needed to permit early intrapartum antibiotic prophylaxis. This study aimed to evaluate the PCR assays targeting the 16S ribosomal RNA gene (16S rDNA) for detection of the GBS in comparison with a specific culture method. Materials and Methods Two swabs were used to obtain ...
full textEpidemiologic Pattern of Vaginal Colonization by Group B Streptococcus in Pregnant Women in Hamadan, Central West of Iran
Group B Streptococcus (GBS) is a leading cause of serious neonatal infections. Although great progress has been made in preventing prenatal GBS, its colonization rate in different regions ofIran remains unknown. The objective of this study was to determine the colonization rate of GBS in pregnant women in Hamadan city, Central west of Iran. A group of 544 pregnant women were randomly selected a...
full textThe Prevalence of Group B Streptococcus Colonization in Iranian Pregnant Women and Its Subsequent Outcome
full text
Prevalence and significance of vaginal group B streptococcus colonization in pregnant women from Osijek, Croatia.
The aim of the study was to determine the prevalence of vaginal group B streptococcus (GBS) colonization in pregnant women from Osijek area, the possible effect of GBS colonization on pregnancy outcome and neonatal complications and the role of intrapartum prophylaxis in this context. This retrospective case-control study took place at the Department of Gynecology and Obstetrics, Osijek Univers...
full textepidemiologic pattern of vaginal colonization by group b streptococcus in pregnant women in hamadan, central west of iran
group b streptococcus (gbs) is a leading cause of serious neonatal infections. although great progress has been made in preventing prenatal gbs, its colonization rate in different regions ofiran remains unknown. the objective of this study was to determine the colonization rate of gbs in pregnant women in hamadan city, central west of iran. a group of 544 pregnant women were randomly selected a...
full textMy Resources
Save resource for easier access later
Journal title:
iranian journal of basic medical sciencesجلد ۱۳، شماره ۴، صفحات ۱۸۳-۱۸۸
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023